Optimization of a Loop-Mediated Isothermal Amplification Assay for On-Site Detection of Fusarium fujikuroi in Rice Seed

Fusarium fujikuroi, causing bakanae disease, is one of the most important seedborne pathogens rice, detection which paramount for seed certification and preventing field infections. Molecular tests—qPCR loop-mediated isothermal amplification (LAMP)—are replacing blotter test in health procedures, due to higher sensitivity, specificity, fast turnaround results delivery, on-site application possibility quantifying endophytic A LAMP test, had been previously developed with primers designed target elongation factor 1-α sequence F. was validated according international validation standard (EPPO, PM7/98) on thirty-four rice lines different levels susceptibility thus comparing it two DNA extraction procedures. The use crude extracted provided more sensitive than commercial kit Omega E.Z.N.A® Plant kit. showed that infection fujikuroi essential development disease minimum amount pathogen necessary corresponds 4.17 × 104 cells/µL. This study confirms applicability technique seeds quantitative pathogen.